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品系全名:C57BL/6JSmoc-Tg(CAG-LSL-dCas9-SPH)Smoc
目錄號:NM-TG-00025
品系狀態(tài):活體
Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, 3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, A-11034, Thermo Fisher).
基因名:dCas9
基因曾用名:Null
NCBI ID:Null
MGI ID:Null
Ensembl ID:Null
Pubmed:dCas9
暫無數(shù)據(jù)
1.具體的試驗周期以工程師告知的為準。
2.文章中的圖片或者標準以及具體的試驗方案僅供參考,因為每個樣品和項目都有所不同,所以最終以工程師告知的為準。
3.關(guān)于(樣品量)的需求,最好是先咨詢我們的工程師確定,避免不必要的樣品損失。
4.加急試驗周期一般是五個工作日左右,部分樣品有所差異
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